Cleavable trifunctional biotin reagents for protein labelling, capture and release.
نویسندگان
چکیده
Trifunctional biotin reagents incorporating cleavable linkers are evaluated for their usage in protein enrichment. A linker based on the Dde protecting group leads to efficient release of protein targets under mild conditions. It additionally contains a masked trypsin cleavage site, which eliminates the majority of the tag during tryptic digestion.
منابع مشابه
A Simple and Effective Cleavable Linker for Chemical Proteomics Applications*□S
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 47 شماره
صفحات -
تاریخ انتشار 2013